Immunofluorescent analysis of FEN1 in Hela cells using a FEN1 monoclonal antibody (Product # MA5-33136) at a dilution of 1:25. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L). Cells were counter-stained with DAPI.